What is how to take vbg from butterfly?

To take a venous blood gas (VBG) from a butterfly catheter in a butterfly, it's crucial to follow a careful procedure to ensure accurate results and minimize trauma to the animal. Here's a breakdown of the key steps:

• Preparation: Gather your necessary supplies: a butterfly catheter (appropriate gauge for the butterfly), a blood gas syringe (pre-heparinized), sterile gauze, alcohol or chlorhexidine swabs, and gloves. Ensure the syringe's heparin is appropriately distributed, as excess heparin can alter blood gas values.

• Site Selection: The site should be easily accessible and have visible superficial veins.Common venipuncture sites in butterflies are the cephalic (foreleg) and saphenous (hindleg) veins. Choose the vein based on size and accessibility.

• Venipuncture: Shave the area if needed. Aseptically prepare the skin with alcohol or chlorhexidine scrub, working in a circular motion from the center outward. Stabilize the vein by applying gentle pressure proximally. Insert the butterfly needle bevel up into the vein at a shallow angle. Once you see blood flashback into the catheter hub, advance the catheter a short distance further into the vein.

• Blood Collection: Attach the pre-heparinized blood gas syringe to the butterfly catheter. Gently aspirate the blood, being careful to avoid creating a vacuum in the syringe which could hemolyze the blood. Collect the appropriate volume of blood for the analyzer being used. If multiple samples are needed, take the blood gas sample first.

• Post-Collection: After obtaining the sample, stop aspirating and remove the syringe while holding pressure on the venipuncture site with sterile gauze. Immediately apply pressure to the puncture site for several minutes to prevent hematoma formation. Gently rock the syringe to mix the blood with the heparin.

• Sample Handling: Remove any air bubbles from the syringe. Cap the syringe securely and label it with the butterfly's identification, date, and time of collection. Transport the sample to the blood gas analyzer immediately or place it on ice if analysis will be delayed (but analyze as soon as possible).

• Butterfly Care: Monitor the butterfly for signs of bleeding or swelling at the venipuncture site. If any complications arise, address them promptly.

• Important Considerations:

  • **Minimizing Stress:**Handle the butterfly gently and calmly to minimize stress, as stress can alter blood gas values.
  • Avoiding Air Contamination: Prevent air from entering the syringe during collection, as air can affect the partial pressure of oxygen (PaO2) and partial pressure of carbon dioxide (PaCO2) values.
  • Hemolysis: Avoid traumatic venipuncture, as this can cause hemolysis, which interferes with accurate blood gas measurements.
  • Heparinization: Use the appropriate amount of heparin to prevent clotting, as excessive heparin can affect the pH and electrolyte values.

Here is the subjects with their links:

• Venous Blood Gas (VBG)
• Butterfly Catheter
• Blood Gas Syringe
• Venipuncture
• Hemolysis
• Heparinization